Journal: Cancer Science

Article Title: Suppression of lymph node and lung metastases of endometrial cancer by muscle‐mediated expression of soluble vascular endothelial growth factor receptor‐3

doi: 10.1111/cas.12184

Figure Lengend Snippet: Suppression of vascular endothelial growth factor (VEGF)‐C‐driven lymphatic endothelial cell (LEC) proliferation by conditioned medium of soluble vascular endothelial growth factor receptor‐3 (sVEGFR‐3)‐expressing cells. Cells were plated at 5 × 103 cells/well in 96‐well plates, and 50% sVEGFR‐3‐conditioned medium or luciferase‐conditioned medium was added with 100 ng/mL recombinant human VEGF‐C. The number of LEC with 50% sVEGFR‐3‐conditioned medium (b) was clearly smaller than that with control (a). The cells were counted by colorimetric assay 48 h after plating. Each bar represents the mean ± SD. (*P < 0.01) (c).

Article Snippet: Human neonatal dermal lymphatic endothelial cells (LEC) were purchased from AngioBio (Del Mar, CA, USA) and maintained in EGM‐MV2 BulletKit (Cambrex, East Rutherford, NJ, USA) supplemented with 10% inactivated FCS at 37°C in a 5% CO 2 atmosphere.

Techniques: Expressing, Luciferase, Recombinant, Colorimetric Assay